We decided to recreate the internal standard because we decided that the solution might need to be refrigerated. We ran a sample of this internal standard in the HPLC, but just like last time, the peaks were not very clear. We wrote a procedure for the HPLC for future use.
Instead of getting more flustered about the HPLC not producing any good spectrum from our samples, we decided to move on to running a sequence of the individual standards of each acid. This sequence will be finished running tomorrow.
On the GCMS, a five sample sequence was run to try and clear out the machine. However, the concentrations of each acid increased with each run. This really makes no sense to anyone, unless the solvent is knocking off more and more of the residual acids. We may try to fix the leak in the other GCMS so we don't have to wait longer to start running actual samples instead of just solvent. A procedure was also written for the GCMS.
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