Today marked the end of the first week in the lab.
We attempted to shorten the gradient on the HPLC after looking at the previous day's run. We started from the conditions at time=30 minutes, where methanol and buffer concentration were both 50%. The run was also extended to 70 minutes. After letting these settings run, the resulting graph showed that we pretty much messed up. The peaks were not clear like the last run.
We tried attempting a few different gradients with different conditions, but each time, the graph seemed to show worse and worse results.
On the GCMS, we ran another sample of 2:1 chloroform:methanol to clean out the machine. The peaks decreased from the previous run, so it seems like the machine is getting clean!
We ran another sample on the GCMS, and, unfortunately, the peaks were higher, indicating that the machine was not clean.
Today was a little disheartening because we could not get any consistent good results, but hopefully the next samples will produce better results!
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