After examining the data from last night's sequence, we decided that a ramp of 15 degrees celsius per minute is the best. This method has a better peak shape and has a faster run time.
We decided to remove stearic acid from our research because we believe it is causing trouble in reading other peaks and we cannot get a good shape on the stearic acid peak.
We set up an experiment with different flow rates to see if that would help separate the oleic and linoleic peaks. We also changed our method to have a solvent delay of 8 minutes to create a better baseline in the chromatogram.
We also are running a sequence with different types of oil/saliva samples:
1. saliva + water
2. water + oil
3. saliva + oil
This is done with corn, olive, and mineral oil.
From our past runs on internal standards, we were able to calculate some peak areas and retention times for different acids that were broken down. With this information, we were able to calculate the response factor for the different acids.
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