I feel like today good progress was made for our research.
We made an internal standard solution with oleic, nonadecanoic, stearic, palmitic, and linoleic acid. Each acid had a concentration of 0.003 M in 100 mL of solution.
The internal standard was run in the HPLC. Ideally, we would get five peaks showing the five different components we put in the solution, but we actually got seven peaks. Some of the acids may be contaminated. To be able to determine which peak is which, individual solutions of each acid needs to be prepared and then run through the HPLC. Only the peaks from that acid will show up on that run.
We made the standards for each individual acid today and will run them tomorrow in the HPLC.
We also discussed what our goals were for tomorrow and how we would accomplish them, including shortening the gradient time on the HPLC, how to calculate the response factor for each acid, and how to calculate the concentration of each acid in a saliva sample.
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