The first thing we did today was look at all of the data from the sequence that ran last night. None of the standards really turned out the way we thought they would, and they all really looked the same. All of the runs on the saliva looked good, however.
After looking at all of the data from the different methods, we decided that the method that ramped up the temperature at 10 degrees Celsius per minute looked the best. We think more experimenting with different methods could produce better results, so we started to prepare for new methods.
We created a new internal standard mix with a higher concentration (200 ppm) of nonadecanoic acid than before. We created five individual standards with each individual acid. A new saliva sample was created. We prepared samples for the GC/MS for each of the previously mentioned and additionally GC/MS samples of pure hexane and 2:1 chloroform:methanol.
We set up the sequence the run with ramp temperatures of 15, 20, and 30 degrees celsius per minute. Hopefully by tomorrow we will be able to tell which ramp rate is the best, and then we will work on figuring out the optimal flow rate of helium through the machine.
No comments:
Post a Comment