Day 8

I think the biggest success of the day was the next attempt at saliva samples.  My sample turned out the clear yellowish color we have been looking for, as did Neelam's!  This is great news.  Whenever we are finally ready to start running our own samples in the GCMS and HPLC, we will have mastered the technique to producing the saliva samples.

We also made another liter of the phosphate buffer (~pH 3) for the HPLC to use.

We ran a sample of the new 0.01 M internal standard on the HPLC.  There were finally some peaks that showed up, but there was a bad baseline on the chromatogram.  I am interested to see how the chromatogram of another sample of this standard will turn out.

Tomorrow, hopefully we will really get going on the GCMS.  We are going to change our solvent for our standard to hexane.  What we want to see with our runs is three good chromatograms with nicely shaped peaks and then we can move on to running our own samples.  Hope we can knock that out tomorrow!