The results on the HPLC showed that there are three consistent small peaks downfield. This means that something is stuck in the machine, but we may be able to just run our samples and subtract out those three peaks that keep showing up. We did run the other half of the sequence on the HPLC because the first half did not show expected peaks.
To try and get peaks to show up, we created a higher concentration internal standard: 0.01 M instead of 0.003 M.
We ran another sequence of 2:1 chloroform:methanol on the GCMS. We also got the older GCMS to work and successfully ran an instrument run (no blank or sample) and a blank run (2:1 chloroform:methanol) without getting any peaks. We ended our work on this GCMS by running a 11.46 ppm sample of our internal standard.
The last thing we worked on was our saliva samples. The first ones did not separate in the separatory funnel like they are supposed to. We believe this is because we agitated the solutions too much. The second ones we made separated much better and formed a clear layer at the bottom, which is what we are looking for. After filtering them, they are not as clear as what we would like, but sometimes they become more clear with time. Regardless, these samples were much better than the first ones we did, and hopefully we will continue to see improvement with them as we do more.
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